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Protocols
Histochemical Staining
Description:
 The staining method involves application of the basic dye haematoxylin, which colours basophilic structures with a blue-purple hue, and alcohol-based acidic eosin Y, which colours eosinophilic structures a bright pink.
The eosinophilic structures are generally composed of intracellular or extracellular protein. The Lewy bodies and the Mallory bodies are examples of eosinophilic structures. Most of the cytoplasms are eosinophilic.
Methods:
Histochemical Staining: there are different main methods used in our labs when performing Histochemical Staining. They are:
Haematoxylin & Eosin (H&E): (click links to download files)
Basic Staining With Haematoxylin
Masson's Trichrome: (click links to download files)
Accustain Trichrome Stains
Masson's Trichrome Technique
Van Gieson: (click links to download files)
Van Gieson Technique
Van Gieson's Picrofuchsin
Movie:
Not Available
More Information:
Haematoxylin & Eosin (H&E) is the most commonly used technique in animal histology and routine pathology. It is used to stain cell nuclei prior to examination under a microscope. The basic dye haematoxylin stains acidic structures a purpilish-blue while Eosin, an acidic dye, stains basic structures red or pink.
Masson's Trichrome is a tissue technique that is used to demonstrate connective tissue elements, principally collagen. The staining technique produces three colours; nuclei and other basophilic structures are stained blue, collagen is stained green or blue depending on which variant of the technique is used, and cytoplasm, muscle, erythorcytes and keratin are stained bright red.
Van Geison is another connective tissue method in which collagen is stained red, nuclei blue and red cells and cytoplasm yellow. When used in combination with an elastic stain, elastin is stained blue/black in addition to the results described above. This staining technique is particularly useful for blood vessels and skin.
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