Introduction:

Main

Biography: Basic Info

Bio-Sketch

Research Interests:
  - Allergic Rhinitis
  - Asthma Research
  - Atopic Dermatitis
  - COPD
  - Berylliosis

Protocols:
  - Immunocytochemistry
  - In Situ Hybridization
  - Western Blot
  - Histochemical Staining
  - Tissue Culture
  - Flow Cytometry
  - Genetic Cloning
  - LCM
  - PCR & Real-Time QPCR
  - Tissue Engineering
  - Antisense Technology
  - DNA Purification

Staff:
  - Research Technicians
  - Fellows
  - Students
  - Alumni

Curriculum Vitae:
  - Personal Information
  - Appointments
  - Past Appointments
  - Research Experience
  - Teaching Experience
  - Organized Courses
  - Administration
  - Memberships
  - Awards
  - Visiting Professor
  - Meetings
  - Editiorial Board

Publications:
  - 2005-Present
  - 2003-2004
  - 2000-2002
  - 1995-1999
  - 1990-1994
  - 1985-1989

Fellowships

Contact Us

 


Protocols


Immunocytochemistry (ICC)


Description:

click here to view larger version. click here to view larger version. Immunocytochemistry (ICC) is used to determine the expression of molecules in cells on slide preparations. Slide preparations can be tissue sections from frozen or paraffin embedded tissues or cytospins. This technique involves the use of antibodies to bind the epitope on the slide. Other antibodies linked to enzymes are added which bind to the first antibody forming a large complex over the epitope. A substrate for the enzyme is applied and a colored product is formed marking the positive cells. The slides can be analyzed by light microscopy. Different variations exist like APAAP and Immunoperoxidase assays.



Methods:

Immunoenzymatic staining methods utilize enzyme-substrate reactions to convert colorless chromogens into colored and products. The main methods used in our lab are:

APAAP: (click links to download files)

Buffers for Immunostaning APAAP and ABC
For Acetone-Methanol Fixed Slides (Tissue & Cytospin)

Immunoperoxidase: (click links to download files)

Immunoperoxidase Protocol for IHC
Methology for Antigen Retrieval

Frozen: (click links to download files)

Tissue Collection for ICC of Biopsy Samples

Paraffin Blocks: (click links to download files)

Human Tissue Collection for ICC



Movie:

Click HERE to view a flash movie (opens in a new window).