Protocols

In Situ Hybridization (ISH)

Description:

In Situ Hybridization (ISH) is a process that localizes or combines a complementary DNA or RNA tissue into a portion or section of a tissue (in situ).

Methods:

In Situ Hybridization: the main methods used in our lab are:

Radioactive Using S35: (click links to download files)

Labelling of RNA Probes by In Vitro Transcription
Prehybridization & Hybridization
Post-Hybridization
Autoradiography of Slides
Basic Solutions To Be Used for ISH
Stock Solutions

Non-Radioactive Using Digoxigenin: (click links to download files)

In Vitro Transcription Using Digoxigenin-UTP
Prehybridization & Hybridization Using DIG.Labeled Probes

Frozen: (click links to download files)

Tissue Collection for ISH of Biopsy Samples

Paraffin Blocks:

Human Tissue Collection for ISH

Movie:

Not Available