Introduction:

Main

Biography: Basic Info

Bio-Sketch

Research Interests:
  - Allergic Rhinitis
  - Asthma Research
  - Atopic Dermatitis
  - COPD
  - Berylliosis

Protocols:
  - Immunocytochemistry
  - In Situ Hybridization
  - Western Blot
  - Histochemical Staining
  - Tissue Culture
  - Flow Cytometry
  - Genetic Cloning
  - LCM
  - PCR & Real-Time QPCR
  - Tissue Engineering
  - Antisense Technology
  - DNA Purification

Staff:
  - Research Technicians
  - Fellows
  - Students
  - Alumni

Curriculum Vitae:
  - Personal Information
  - Appointments
  - Past Appointments
  - Research Experience
  - Teaching Experience
  - Organized Courses
  - Administration
  - Memberships
  - Awards
  - Visiting Professor
  - Meetings
  - Editiorial Board

Publications:
  - 2005-Present
  - 2003-2004
  - 2000-2002
  - 1995-1999
  - 1990-1994
  - 1985-1989

Fellowships

Contact Us

 


Protocols


PCR & Real-Time QPCR


Description:

click here to view larger version. click here to view larger version. Polymerase Chain Reaction (PCR) is a method for amplifying a DNA base sequence using a heat-stable polymerase and two 20-base primers. One complementary is attached to the postive strand to be amplified and the other complementary is attached to the negative strand. Because the newly synthesized DNA strands can subsequently serve as additional templates for the same primer sequences, successive rounds of primer annealing, strand elongation and dissociation produce rapid and highly specific amplifications of the desired sequence. PCR can also be used to detect the existence of the defined sequence in a DNA sample.



Methods:

Reverse Transcription Protocol (step before PCR)

Real-Time PCR Protocol
Classic PCR Protocol



Movie:

Click HERE to view a flash movie (opens in a new window).