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Protocols
Western Blot
Description:
 Western Blot is a method used in molecular biology/biochemistry/immunogenetics to detect protein in a given sample of tissue homogenate or extract. It uses gel electrophoresis to separate denatured proteins by mass. The proteins are then transferred out of the gel and onto a membrane (typically nitrocellulose), where they are "probed" using antibodies specific to the protein. As a result, researchers can examine the amount of protein in a given sample and compare levels between several groups. Other techniques also using antibodies allow detection of proteins in tissues (immunohistochemistry) and cells (immunocytochemistry).
Credit Source: Wikipedia.
Methods:
Quantification of Proteins for Western Blot
Western Blot Recipes
Protein Extraction for Western Blot
Western Blot Instructions
Movie:
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